Comparison of aerobic methanotrophic communities in littoral and profundal sediments of Lake Constance by a molecular approach.
نویسندگان
چکیده
Analysis of pmoA and 16S rRNA gene clone libraries of methanotrophic bacteria in Lake Constance revealed an overall dominance of type I methanotrophs in both littoral and profundal sediments. The sediments exhibited minor differences in their methanotrophic community structures. Type X methanotrophs made up a significant part of the clone libraries only in the profundal sediment and were also found only there as a prominent peak by T-RFLP analyses.
منابع مشابه
Aerobic methanotrophic bacterial communities in sediments of Lake Constance
25 Introduction 26 Materials and Methods 28 Results 36 Discussion 47 3. Characterization of a novel methanotroph, Methylosoma difficile gen. nov., sp. nov., enriched by gradient cultivation from littoral sediment of Lake Constance 55 Summary 55 Introduction 57 Methods 58 Results and 64 Discussion 4. Comparison of aerobic methanotrophic communities in littoral and profundal sediment of Lake Cons...
متن کاملActivity and diversity of methanotrophic bacteria at methane seeps in eastern Lake Constance sediments.
The activity and community structure of aerobic methanotrophic communities were investigated at methane seeps (pockmarks) in the littoral and profundal zones of an oligotrophic freshwater lake (Lake Constance, Germany). Measurements of potential methane oxidation rates showed that sediments inside littoral pockmarks are hot spots of methane oxidation. Potential methane oxidation rates at littor...
متن کاملpmoA-based analysis of methanotrophs in a littoral lake sediment reveals a diverse and stable community in a dynamic environment.
Diversity and community structure of aerobic methane-oxidizing bacteria in the littoral sediment of Lake Constance was investigated by cloning analysis and terminal restriction fragment length polymorphism (T-RFLP) fingerprinting of the pmoA gene. Phylogenetic analysis revealed a high diversity of type I and type II methanotrophs in the oxygenated uppermost centimeter of the sediment. T-RFLP pr...
متن کاملAbundance and activity of methanotrophic bacteria in littoral and profundal sediments of lake constance (Germany).
The abundances and activities of aerobic methane-oxidizing bacteria (MOB) were compared in depth profiles of littoral and profundal sediments of Lake Constance, Germany. Abundances were determined by quantitative PCR (qPCR) targeting the pmoA gene and by fluorescence in situ hybridization (FISH), and data were compared to methane oxidation rates calculated from high-resolution concentration pro...
متن کاملMicroelectrode measurements of nitrate gradients in the littoral and profundal sediments of a meso-eutrophic lake (lake vechten, the Netherlands).
NO(3) concentration profiles were measured in the sediments of a meso-eutrophic lake with a newly developed microelectrode. The depth of penetration of NO(3) varied from only 1.3 mm in organic-rich profundal silty sediments to 5 mm in organic-poor littoral sandy sediments. The thickness of the zone of denitrification in the organic-rich sediments was <500 mum. Oxygen profiles measured simultane...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Applied and environmental microbiology
دوره 73 13 شماره
صفحات -
تاریخ انتشار 2007